Determination of Dose-depth Distribution of Proton Beam Using Resazurin Metabolism Assay
(KIM Min Jung, PAL Sukdeb, SONG Joon Myong
,Vol(8),Pages(317-317),,, Year: 2008)
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No information is available
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Removal of Herbicides Using Surface Engineered Carbon
(LEE Su Chul, PAL Sukdeb, SONG Joon Myong,Vol(8),Pages(317-317),,, Year: 2008)
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No information is available
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Nanocrystalline-Silver Impregnated Activated Carbon Matrix with High Activity Antibacterial
(PAL Sukdeb, SONG Joon Myong
,Vol(8),Pages(206-206),,, Year: 2008)
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No information is available
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Electrochemiluninesce-based DNA Detection Using Microelectrode Array
(PAL Sukdeb, SONG Joon Myong,Vol(8)Pages(312-312),,, Year: 2008)
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No information is available
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Microelectrode array for electrochemiluminescence-based DNA detection (Biochip Journal)
(Sukdeb Pal, Min Jung Kim, Joon Myong Song,Vol(2),Issue(1),Pages(66-72),,, Year: 2008)
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No information is available
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Quantitation of surface coverage of oligonucleotides bound to chip surfaces: a fluorescence-based approach using alkaline phosphatase digestion (Lab on a Chip)
(Sukdeb Pal, Min Jung Kim, Joon Myong Song,Vol(8),Issue(8),Pages(1332-1341),,, Year: 2008)
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Silanized chip surfaces provide a reliable substrate for immobilization of oligonucleotides. The ability for rapid and sensitive detection of oligonucleotide surface coverage on these chips is crucial for their wide and effective applications in biotechnology. In this paper, two different silanization procedures were used to covalently bind fluorescent-labeled single-stranded DNA onto silicon dioxide or nitride chip surfaces. Effects of surface functionalization techniques for different surfaces, and immobilization conditions, including buffers and solution ionic strength, on surface probe coverage were investigated, quantifying the endpoint probe density by fluorescent measurement upon digestion with alkaline phosphatase (ALP). Digestion of surface-immobilized oligonuleotides with ALP released the fluorophore-tagged probe fragments back into the solution.
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Concentration and recovery of coliphages from water with bituminous coal (Water Environmental Research)
(Nishant Dafale, Sukumar Lakhe, K. Yadav, Hemant Purohit & Tapan Chakraborty
,80 3,,282-288, Year: 2008)
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Coliphages represent a process indicator for fecal pollution. The coal bed concentration method prepared for enterovirus was refined for a concentration of coliphages. A bed made from 1.5 g of 120-mesh coal powder was used for concentrating coliphage from 200 mL of a water sample with or without the addition of aluminum chloride at different pH values. The isolated E. coli strain EC-R8 was found to be more susceptible to the desired coliphage and showed significant coliphage-coliform response, with clear plaque used for further studies. The complete coliphage adsorption was achieved with the addition of 0.0005 M AICI3 at pH 6.0. Adsorbed coliphages were eluted with 3% beef extract in Mcllvaine buffer at pH 7.1, with an average recovery of 78.74%. This concentration technique was applied for the detection of coliphages from the well water of Nagpur city (India) and found to contain coliphages in the range of 2 to 28 plaque-forming units per liter (PFU/L). Water Environ. Res.,
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Decolorization of azo dyes and simulated dye bath wastewater using acclimatized microbial consortium?biostimulation and halotolerance (Bioresource Technology)
(Nishant Dafale, Nageshwara Rao, Sudhir Meshram & Satish Wate
,99,,2552-2558, Year: 2008)
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Anaerobic acclimatization of activated sludge from a textile effluent treatment plant to high concentration of RB5 could effectively decolorize RB5 dye solution. The strains viz. Pseudomonas aeruginosa and Bacillus circulans and other unidentified laboratory isolates (NAD1 and NAD6) were predominantly present in the microbial consortium. The conditions for efficient decolorization, biostimulation to increase effectiveness of microbial consortium, its tolerance to high salt concentration and non-specific ability towards decolorization of eight azo dyes, are reported. The optimum inoculums concentration for maximum decolorization were found to be 1–5 ml of 1800 ± 50 mg l1 MLSS and 37 C, respectively. The decolorization efficiency was 70–90% during 48 h. The biomass showed efficient decolorization even in the presence of 10% NaCl, as tested with RB5. In the presence of flavin adenine dinucleotide (FAD) more than 99% decolorization occurred in 8 h. The decolorization of RB5 was traced to extracellular enzymes. The effectiveness of acclimatized biomass under optimized conditions towards decolorization of two types of synthetic dye bath wastewaters that were prepared using chosen azo dyes is reported.
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Biodegradation enhancement of purified terephthalic acid wastewater by coagulation-flocculation process as pretreatment (Journal of Hazardous Material )
(M. Karthik, Nishant Dafale, Praduamya Pathe & Tapas Nandy
,154 1-3,,721-730, Year: 2008)
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In this work, the coagulation–flocculation process was used as pretreatment for purified terephthalic acid (PTA) wastewater with the objective of improving its overall biodegradability. PTA production generates wastewaters with toxicants p-xylene [1,4-dimethyl-benzene (C8H10)], a major raw material used in the production process, along with some of the intermediates, viz., p-toluic acid, benzoic acid, 4-carboxybenzaldehyde, phthalic acid and terephthalic acid. These compounds affect the bio-oxidation process of wastewater treatment; hence removal of these constituents is necessary, prior to conventional aerobic treatment. This paper addresses the application of coagulation–flocculation process using chemical coagulants, viz., aluminium sulphate (alum), polyaluminium chloride (PAC), ferrous sulphate and ferric chloride in combination with anionic polyelectrolyte. Polyaluminium chloride (PAC) in conjunction with lime and polyelectrolyte removed about 63.1% chemical oxygen demand (COD) and 45.2% biochemical oxygen demand (BOD) from PTA wastewater. Coagulation–flocculation process coupled with aerobic bio-oxidation treatment of PTA wastewater achieved, COD & BOD removals of 97.4% and 99.4%, respectively. The biodegradability enhancement evaluated in terms of the BOD5/COD ratio, increased from 0.45 to 0.67 at the optimum conditions. The results obtained from these studies indicate that the coagulation–flocculation process could be a suitable pretreatment method in reducing toxicity of PTA wastewater whilst enhancing biodegradability for aerobic biological treatment scheme.
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Kinetic study approach of remazol black-B use for the development of two-stage anoxic-oxic reactor for decolorization/biodegradation of azo dyes by activated bacterial consortium (Journal of Hazardous Material )
(Nishant Dafale, Satish Wate, Sudhir Meshram & Tapas Nandy
,159 ,,319-328, Year: 2008)
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The laboratory-isolated strains Pseudomonas aeruginosa, Rhodobacter sphaeroides, Proteus mirabilis, Bacillus circulance, NAD 1 and NAD 6 were observed to be predominant in the bacterial consortium responsible for effective decolorization of the azo dyes. The kinetic characteristics of azo dye decolorization by bacterial consortium were determined quantitatively using reactive vinyl sulfonated diazo dye, remazol black-B (RB-B) as a model substrate. Effects of substrate (RB-B) concentration as well as different substrates (azo dyes), environmental parameters (temperature and pH), glucose and other electron donor/co-substrate on the rate of decolorization were investigated to reveal the key factor that determines the performance of dye decolorization. The activation energy (Ea) and frequency factor (K0) based on the Arrhenius equation was calculated as 11.67 kcal mol−1 and 1.57 × 107 mg l g MLSS−1 h−1, respectively. The Double-reciprocal or Lineweaver–Burk plot was used to evaluate Vmax, 15.97 h−1 and Km, 85.66 mg l−1. The two-stage anoxic–oxic reactor system has proved to be successful in achieving significant decolorization and degradation of azo dyes by specific developed bacterial consortium with a removal of 84% color and 80% COD for real textile effluents vis-a-vis ` ≥90% color and COD removal for synthetic dye solution
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GIS for Municipal Solid Waste Management (Journal of Indian Association for Environment Management)
(Ritesh Vijay and Apurba Gupta,35,1,20-22, Year: 2008)
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No information is available
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GIS-based locational analysis of collection bins in municipal solid waste management systems ( Journal of Environmental Engineering and Science)
(Ritesh Vijay, Ajay Gautam, Ajay Kalamdhad, A. Gupta S. Devotta,7,1,39-43, Year: 2008)
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Solid waste management systems currently receive wide attention, from both economic and environmental plan-ners, because of their complexity during coordination of various management strategies. The efficiency and cost effective-ness of route optimization and disposal site selection depend largely upon the appropriate placement of storage bins and their corresponding command area for waste contribution. The present paper illustrates a geographic information system (GIS) based algorithm for optimal location and number of storage bins, considering p-median constrained model, based on Indian guidelines for Municipal Solid Waste Rules. The algorithm also computes the contributing command area of ??solid waste to a particular bin, based on the shortest distance, with descending slope for ease in solid waste collection. Summary : Solid waste management systems are currently attracting much attention from economic and environmental planners due to their complexity in coordinating various management strategies. The efficiency and cost-effectiveness of route optimization and landfill site selection largely depend on the appropriate location of the storage bins and their corresponding service area for waste collection. This article presents an algorithm based on a Geographic Information System (GIS) used to determine the optimal location and number of storage bins, given a constrained p-median model, based on guidelines for the regulation of municipal solid waste in India. The The algorithm also calculates the solid waste collection area served by a particular storage bin based on the shortest distance, with a downward slope to facilitate solid waste collection. Keywords: municipal solid waste, location of bins, area served, geographic information system.
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Correlating on- line monitoring parameters, pH, DO and ORP with nutrient removal in an intermittent cyclic process bioreactor system (Bio-Resource Technology)
(Pankaj Tanwar, Tapas Nandy, Pallavi Ukey, Pravin Manekar,99,16,7630-7635, Year: 2008)
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The paper presents the study correlating the profile of on-line monitoring parameters and nutrient removal in an intermittent cyclic process bioreactor (ICPBR) system, thereby utilizing the parameters as operational tool. A laboratory scale ICPBR was employed to treat low C/N ratio domestic wastewater from a township. The study was conducted for correlating biological nutrient removal and on-line monitored parameters pH, dissolved oxygen (DO) and oxidation–reduction potential (ORP). The results revealed that pH, DO and ORP related with the dynamic behavior of nutrient concentration (NH4–N, NO3–N, and PO4–P) during treatment in an ICPBR system. The variation in pH and ORP of the reactor liquor correlate to conversion of ammonia (NH4–N) and nitrate (NO3–N) concentrations, respectively. As the bioconversion of ammonia nitrogen and phosphorus are related to the varying profile of the on-line monitored parameters, the profiles could possibly be used as onsite process control parameters.
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Water conservation through implementation of ultrafiltration and reverse osmosis system with recourse to recycling of effluent in textile industry (Resources, Conservation and Recycling)
(Tapas Nandy, Pravin Manekar, Rita Dhodapkar, Girish Pophali, Sukumar Devotta,Vol(51) Issue(1) Pages(64-77),,, Year: 2007)
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In compliance with the protection of the drinking water source in one of the state capital of India, a cluster of small scale textile industries implemented membrane processes based on treatability studies for recovery of boiler feed water quality with recourse to effluent recycling/reuse. The paper addresses to a case study in one of the textile units on upgradation of a full scale effluent treatment plant comprising chemical, biological, tertiary and advanced treatment processes. Based on the adequacy assessment of chemical and biological processes, improvement in the performance of the unit processes were achieved through optimization of coagulant dosage for chemical coagulation and build-up of active biomass in the activated sludge system. In addition, application of membrane separation processes comprising ultrafiltration and reverse osmosis units are also highlighted including disposal of reverse osmosis (RO) rejects through evaporator leading to zero liquid effluent discharge. The treatment scheme implemented resulted in conservation of around 55% of fresh water demand for industry.
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Direct Evidence for Shape Dependent Antibacterial Activity of Silver Nanoparticles
(TAK YU KYUNG, PAL SUKDEB, SONG JOON MYONG,Pages(332-332),,, Year: 2007)
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No information is available
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Single Nucleotide Polymorphism (SNP) Analysis Based on Capillary Electroch romatography (CEC) using Colloidal Poly (N-isopropylacrylamide) Particles as Pseudostationary Phase
(PAL SUKDEB, SONG JOON MYONG,Pages(397-397),,, Year: 2007)
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No information is available
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Miniaturized DNA Chip Based on Bipolar Semiconductor Technology
(PAL SUKDEB, KIM MIN JUNG, SONG JOON MYONG,Pages(399-399),,, Year: 2007)
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No information is available
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Low Noise Bipolar semiconductor Protein Chip for On-Chip Detection ofE. coliO157: H7
(KIM MIN JUNG, PAL SUKDEB, TAK YU KYUNG SONG JOON MYONG,,,, Year: 2007)
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No information is available
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Integrated circuit bipolar semiconductor microchip-based optical detection of biomolecules: Parallel applications as a miniaturized sample platform and a two-dimensional photodetector (Biochip Journal)
(Sukdeb Pal, Joon Myong Song,Vol(1),Issue(2),Pages(124-133),,, Year: 2007)
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Recently, our laboratory developed a photodiode array (PDA) microchip based on bipolar semiconductor technology. A 12 x 12 micro array of photodiodes, an array of current amplifiers, and a photodiode element-addressing circuit were integrated into a single IC chip. Each photodiode had dimensions of 300 x 300 mu m(2) and the photodiode-to-photodiode distance was 100 gm. The chip was successfully applied to the on-chip detection of target DNA and protein based on optical transduction detection method. This report demonstrates the compatibility of the constructed PDA chips to the on-chip reaction conditions and the effects of operational parameters that may lead to false data interpretation. The chip has also been used as a two dimensional photodetector in a compact optical system to detect Escherichia coli O157: H7 based on the combined use of ELISA and laser-induced fluorescence (LIF).
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Determination of the dose (Analytica chimica acta)
(Min Jung Kim, Sukdeb Pal, Yu Kyung Tak, Kyeong-Hee Lee, Tae Keun Yang, Su-Jae Lee, Joon Myong Song,Vol(593),Issue(2),Pages(214-223),,, Year: 2007)
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In this study the dose?depth distribution pattern of proton beams was investigated by inactivation of human cells exposed to high-LET (linear energy transfer) protons. The proton beams accelerated up to 45 MeV were horizontally extracted from the cyclotron, and were delivered to the cells acutely through a home made prototype over a range of physical depths (in the form of a variable water column). The biological systems used here were two in vitro cell lines, including human embryonic kidney cells (HEK 293), and human breast adenocarcinoma cell line (MCF-7). Cells were exposed to unmodulated proton beam radiation at a dose of 50 Gy similar to that used in therapy. Resazurin metabolism assay was investigated for measurement of cell response to irradiation as a simple and non-destructive assay.
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