- 01 October, 2023
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Polymerase chain reaction (PCR) is a technique used in molecular biology to amplify a single copy or a few copies of a segment of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. PCR is now a common and often indispensable technique used in clinical and research laboratories for a broad variety of applications. These include DNA cloning for sequencing, gene cloning and manipulation, gene mutagenesis, construction of DNA-based phylogenies, or functional analysis of genes; diagnosis and monitoring of hereditary diseases; amplification of ancient DNA, analysis of genetic fingerprints for DNA profiling (for example, in forensic science and parentage testing)and detection of pathogens in nucleic acid tests for the diagnosis of infectious diseases.
