Two Stage Biooxidation (TSB)

Two Stage Biooxidation (TSB) Process


The two stage bio-oxidation (TSB) process, a biological wastewater treatment process, has been developed and implemented at NagarjunaAgrichem Limited, Srikakulam (A.P.) to treat any quantity of effluent generated at the industrial site. The TSB process can be adopted to establish a new wastewater treatment facility or implemented to reengineer the existing wastewater treatment facility


Know-how of the TSB process

  1. TSBinvolves a two-stage sequential batch reactor system
  2. Each stage functions as a cyclic activated sludge process comprising filling, aeration, settling and decantation phases
  3. The organic pollutants in the equalized effluent are biooxidised by heterotrophic bacterial strains like Pseudomonas, Bacillus, Paracoccus, etc., including removal of volatile organic compounds (VOCs) and phenols in stage-I
  4. The autotrophic nitrifying bacteria (Nitrosomonas, Nitrobacter, Nitrosococcus, Nitrovibrio, Nitrospira sp. etc) oxidize ammonia present in the effluent in addition to biodegration of residual organics in stage-II
  5. TSB treats high COD and ammonia bearing wastewater through ‘separated heterotrophic-autotrophic reactions’
  6. The treated effluent meets the reverse osmosis feed standards for recycle and reuse (COD < 100 mg/L, BOD < 30 mg/L and NH3-N < 5 mg/L)


Salient features

  • No chemical treatment involved
  • Removal of both organics and ammonia including phenol and cyanide through bio-oxidation process
  • Inhibition of nitrification in presence of organics is eliminated
  • Two-stage separation process targets removal of specific pollutants in each stage through development of specific bacterial strains


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